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cell culture 852 human embryonic kidney hek 293ft cells  (ATCC)


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    ATCC cell culture 852 human embryonic kidney hek 293ft cells
    Cell Culture 852 Human Embryonic Kidney Hek 293ft Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+kidney+293ft+cells/293T/pm42015511-356-2-11
    Average 99 stars, based on 38071 article reviews
    cell culture 852 human embryonic kidney hek 293ft cells - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Annexin A2 Silencing Induces G 2 Arrest of Non-small Cell Lung Cancer Cells through p53-dependent and -independent Mechanisms
    Article Snippet: Cumulative disease-free survival curves for patients were plotted using the Kaplan-Meier method with commercially available statistical software (SPSS 18.0 for Windows; Systat Software, Inc., San Jose, CA). .. Human lung adenocarcinoma A549 (CCL-185, ATCC) and H1975 (CRL-5908, ATCC) cells, human large cell lung cancer H460 cells (HTB-177, ATCC), human cervical cancer SiHa cells (HTB-35, ATCC), human gastric cancer MKN45 cells (JCRB0254, RIKEN Cell Bank, Japan), human embryonic kidney 293FT cells (PTA-5077, ATCC), human cervical carcinoma CaSki cells (CRL-1550, ATCC), and human hepatoma Hep3B (HB-8064, ATCC), and Huh-7 (PTA-4583, ATCC) cells were cultured in Dulbecco's modified Eagle's medium or RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen). ..

    Article Title: Microglia mediated by LAG3 regulate astrocyte morphogenesis in the brain white matter.
    Article Snippet: .. Human embryonic kidney 293FT cells (ATCC, CRL-1573) and mouse neuroblastoma N2a (ATCC, CCL-131) cells were cultured in high-glucose DMEM medium (Gibco,11995-065) supplemented with 10% fetal bovine serum (FBS) (Gibco,16000044), 1% penicillin/streptomycin (Invitrogen, 15070063). .. The mouse primary astrocytes were cultured in DMEMF/12 (Gibco, 11330-032) containing 10% FBS (Gibco,16000044) and 1% penicillin/streptomycin (Invitrogen, 15070063).

    Article Title: Annexin A2 Silencing Induces G2 Arrest of Non-small Cell Lung Cancer Cells through p53-dependent and -independent Mechanisms
    Article Snippet: .. Cell Cultures and Reagents—Human lung adenocarcinoma A549 (CCL-185, ATCC) and H1975 (CRL-5908, ATCC) cells, human large cell lung cancer H460 cells (HTB-177, ATCC), human cervical cancer SiHa cells (HTB-35, ATCC), human gastric cancer MKN45 cells (JCRB0254, RIKEN Cell Bank, Japan), human embryonic kidney 293FT cells (PTA-5077, ATCC), human cervical carcinoma CaSki cells (CRL-1550, ATCC), and human hepatoma Hep3B (HB-8064, ATCC), and Huh-7 (PTA-4583, ATCC) cells were cultured in Dulbecco’s modified Eagle’s medium or RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen). ..

    Article Title: Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning
    Article Snippet: Dulbecco’s modified Eagle medium (DMEM; Sigma, cat. no. D6429) Fetal bovine serum (FBS; Sigma, cat. no. F2442) 1× phosphate-buffered saline (PBS; Gibco, cat. no. 14190-144, or any brand but without calcium and magnesium, 0.01 M, pH = 7.4) Puromycin (InvivoGen, cat. no. ant-pr-1) Hygromycin-B (InvivoGen, cat. no. ant-hg-1) Polyethylenimine (PEI) transfection reagent (Sigma, cat. no. 913375) Polyethylene glycol (PEG)-6000 (Sigma, cat. no. 81260) Sodium chloride (NaCl; Sigma, cat. no. S5886) Sodium butyrate (Sigma, cat. no. B5887-250 MG) 0.5 M EDTA (IBI Scientific, cat. no. IB70184) Polybrene (stock: 10 mg/mL, EMD Millipore, cat. no. TR1003G) Advanced DMEM–F-12 medium (Gibco, cat. no. 12634-010) Penicillin/streptomycin (Invitrogen, cat. no. 15140-122) 1 M HEPES (Invitrogen, cat. no. 15630-080) GlutaMAX-I (Invitrogen, cat. no. 35050-061) 0.05% (wt/vol) trypsin with 0.5 mM EDTA (Gibco, cat. no. 25300-054) TrypLE Express enzyme (1×), no phenol red (Thermo, cat. no. 12604-013) Mouse recombinant epidermal growth factor (Invitrogen, cat. no. PMG8043) Nicotinamide (Sigma, cat. no. N0636) [Leu15]-Gastrin I (Sigma, cat. no. G9145) A-83-01 (Tocris Bioscience, cat. no. 2939) SB202190 (Sigma, cat. no. S7067) 50× B27 supplement (Invitrogen, cat. no. 17502-044) 100× N2 supplement (Invitrogen, cat. no. 17502-048) N -acetylcysteine (Sigma, cat. no. A9165-5G) Y-27632 stock solution (Rock inhibitor, Sigma, cat. no. Y0503–5MG, 5 mM in 1× PBS, working concentration 10 μM) Recovery cell freezing medium (Invitrogen, cat. no. 12648-010) 40 μm cell strainer (Falcon, cat. no. 352340) Puromycin dihydrochloride solution (10 mg/mL in H 2 O, Sigma, cat. no. P9620-10ML) Hygromycin-B solution (50 mg/mL in H 2 O, Sigma, cat. no. H5527-250MG) Sodium glycochenodeoxycholate (Sigma, cat. no. G0759-25MG) Matrigel growth factor reduced basement membrane matrix (Corning, cat. no. 356231) .. Human embryonic kidney 293FT cells (ATCC, cat. no. PTA-5077, RRID: CVCL_6911) L-WRN cells (ATCC cat. no. CRL-3276, RRID: CVCL_DA06) HIEs: J2 jejunal lines obtained from the Digestive Disease Core at BCM (for transduction, the HIEs were cultured in WRNE medium for >4 d for stem cell proliferation) ! ..

    Article Title: Annexin A2 Silencing Induces G 2 Arrest of Non-small Cell Lung Cancer Cells through p53-dependent and -independent Mechanisms
    Article Snippet: .. Cell Cultures and Reagents Human lung adenocarcinoma A549 (CCL-185, ATCC) and H1975 (CRL-5908, ATCC) cells, human large cell lung cancer H460 cells (HTB-177, ATCC), human cervical cancer SiHa cells (HTB-35, ATCC), human gastric cancer MKN45 cells (JCRB0254, RIKEN Cell Bank, Japan), human embryonic kidney 293FT cells (PTA-5077, ATCC), human cervical carcinoma CaSki cells (CRL-1550, ATCC), and human hepatoma Hep3B (HB-8064, ATCC), and Huh-7 (PTA-4583, ATCC) cells were cultured in Dulbecco's modified Eagle's medium or RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen). ..

    Modification:

    Article Title: Annexin A2 Silencing Induces G 2 Arrest of Non-small Cell Lung Cancer Cells through p53-dependent and -independent Mechanisms
    Article Snippet: Cumulative disease-free survival curves for patients were plotted using the Kaplan-Meier method with commercially available statistical software (SPSS 18.0 for Windows; Systat Software, Inc., San Jose, CA). .. Human lung adenocarcinoma A549 (CCL-185, ATCC) and H1975 (CRL-5908, ATCC) cells, human large cell lung cancer H460 cells (HTB-177, ATCC), human cervical cancer SiHa cells (HTB-35, ATCC), human gastric cancer MKN45 cells (JCRB0254, RIKEN Cell Bank, Japan), human embryonic kidney 293FT cells (PTA-5077, ATCC), human cervical carcinoma CaSki cells (CRL-1550, ATCC), and human hepatoma Hep3B (HB-8064, ATCC), and Huh-7 (PTA-4583, ATCC) cells were cultured in Dulbecco's modified Eagle's medium or RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen). ..

    Article Title: Annexin A2 Silencing Induces G2 Arrest of Non-small Cell Lung Cancer Cells through p53-dependent and -independent Mechanisms
    Article Snippet: .. Cell Cultures and Reagents—Human lung adenocarcinoma A549 (CCL-185, ATCC) and H1975 (CRL-5908, ATCC) cells, human large cell lung cancer H460 cells (HTB-177, ATCC), human cervical cancer SiHa cells (HTB-35, ATCC), human gastric cancer MKN45 cells (JCRB0254, RIKEN Cell Bank, Japan), human embryonic kidney 293FT cells (PTA-5077, ATCC), human cervical carcinoma CaSki cells (CRL-1550, ATCC), and human hepatoma Hep3B (HB-8064, ATCC), and Huh-7 (PTA-4583, ATCC) cells were cultured in Dulbecco’s modified Eagle’s medium or RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen). ..

    Article Title: Annexin A2 Silencing Induces G 2 Arrest of Non-small Cell Lung Cancer Cells through p53-dependent and -independent Mechanisms
    Article Snippet: .. Cell Cultures and Reagents Human lung adenocarcinoma A549 (CCL-185, ATCC) and H1975 (CRL-5908, ATCC) cells, human large cell lung cancer H460 cells (HTB-177, ATCC), human cervical cancer SiHa cells (HTB-35, ATCC), human gastric cancer MKN45 cells (JCRB0254, RIKEN Cell Bank, Japan), human embryonic kidney 293FT cells (PTA-5077, ATCC), human cervical carcinoma CaSki cells (CRL-1550, ATCC), and human hepatoma Hep3B (HB-8064, ATCC), and Huh-7 (PTA-4583, ATCC) cells were cultured in Dulbecco's modified Eagle's medium or RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen). ..

    Transduction:

    Article Title: Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning
    Article Snippet: Dulbecco’s modified Eagle medium (DMEM; Sigma, cat. no. D6429) Fetal bovine serum (FBS; Sigma, cat. no. F2442) 1× phosphate-buffered saline (PBS; Gibco, cat. no. 14190-144, or any brand but without calcium and magnesium, 0.01 M, pH = 7.4) Puromycin (InvivoGen, cat. no. ant-pr-1) Hygromycin-B (InvivoGen, cat. no. ant-hg-1) Polyethylenimine (PEI) transfection reagent (Sigma, cat. no. 913375) Polyethylene glycol (PEG)-6000 (Sigma, cat. no. 81260) Sodium chloride (NaCl; Sigma, cat. no. S5886) Sodium butyrate (Sigma, cat. no. B5887-250 MG) 0.5 M EDTA (IBI Scientific, cat. no. IB70184) Polybrene (stock: 10 mg/mL, EMD Millipore, cat. no. TR1003G) Advanced DMEM–F-12 medium (Gibco, cat. no. 12634-010) Penicillin/streptomycin (Invitrogen, cat. no. 15140-122) 1 M HEPES (Invitrogen, cat. no. 15630-080) GlutaMAX-I (Invitrogen, cat. no. 35050-061) 0.05% (wt/vol) trypsin with 0.5 mM EDTA (Gibco, cat. no. 25300-054) TrypLE Express enzyme (1×), no phenol red (Thermo, cat. no. 12604-013) Mouse recombinant epidermal growth factor (Invitrogen, cat. no. PMG8043) Nicotinamide (Sigma, cat. no. N0636) [Leu15]-Gastrin I (Sigma, cat. no. G9145) A-83-01 (Tocris Bioscience, cat. no. 2939) SB202190 (Sigma, cat. no. S7067) 50× B27 supplement (Invitrogen, cat. no. 17502-044) 100× N2 supplement (Invitrogen, cat. no. 17502-048) N -acetylcysteine (Sigma, cat. no. A9165-5G) Y-27632 stock solution (Rock inhibitor, Sigma, cat. no. Y0503–5MG, 5 mM in 1× PBS, working concentration 10 μM) Recovery cell freezing medium (Invitrogen, cat. no. 12648-010) 40 μm cell strainer (Falcon, cat. no. 352340) Puromycin dihydrochloride solution (10 mg/mL in H 2 O, Sigma, cat. no. P9620-10ML) Hygromycin-B solution (50 mg/mL in H 2 O, Sigma, cat. no. H5527-250MG) Sodium glycochenodeoxycholate (Sigma, cat. no. G0759-25MG) Matrigel growth factor reduced basement membrane matrix (Corning, cat. no. 356231) .. Human embryonic kidney 293FT cells (ATCC, cat. no. PTA-5077, RRID: CVCL_6911) L-WRN cells (ATCC cat. no. CRL-3276, RRID: CVCL_DA06) HIEs: J2 jejunal lines obtained from the Digestive Disease Core at BCM (for transduction, the HIEs were cultured in WRNE medium for >4 d for stem cell proliferation) ! ..



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