cell culture 852 human embryonic kidney hek 293ft cells (ATCC)
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cell culture 852 human embryonic kidney hek 293ft cells
Cell Culture 852 Human Embryonic Kidney Hek 293ft Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+embryonic+kidney+293ft+cells/293T/pm42015511-356-2-11
Average 99 stars, based on 38071 article reviews
Cell Culture 852 Human Embryonic Kidney Hek 293ft Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+embryonic+kidney+293ft+cells/293T/pm42015511-356-2-11
Average 99 stars, based on 38071 article reviews
cell culture 852 human embryonic kidney hek 293ft cells - by Bioz Stars,
2026-10
99/100 stars
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Cell Cultures and Reagents Human lung adenocarcinoma A549 (CCL-185, ATCC) and H1975 (CRL-5908, ATCC) cells, human large cell lung cancer H460 cells (HTB-177, ATCC), human cervical cancer SiHa cells (HTB-35, ATCC), human gastric cancer MKN45 cells (JCRB0254, RIKEN Cell Bank, Japan), Transduction:Article Title: Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning Article Snippet: Dulbecco’s modified Eagle medium (DMEM; Sigma, cat. no. D6429) Fetal bovine serum (FBS; Sigma, cat. no. F2442) 1× phosphate-buffered saline (PBS; Gibco, cat. no. 14190-144, or any brand but without calcium and magnesium, 0.01 M, pH = 7.4) Puromycin (InvivoGen, cat. no. ant-pr-1) Hygromycin-B (InvivoGen, cat. no. ant-hg-1) Polyethylenimine (PEI) transfection reagent (Sigma, cat. no. 913375) Polyethylene glycol (PEG)-6000 (Sigma, cat. no. 81260) Sodium chloride (NaCl; Sigma, cat. no. S5886) Sodium butyrate (Sigma, cat. no. B5887-250 MG) 0.5 M EDTA (IBI Scientific, cat. no. IB70184) Polybrene (stock: 10 mg/mL, EMD Millipore, cat. no. TR1003G) Advanced DMEM–F-12 medium (Gibco, cat. no. 12634-010) Penicillin/streptomycin (Invitrogen, cat. no. 15140-122) 1 M HEPES (Invitrogen, cat. no. 15630-080) GlutaMAX-I (Invitrogen, cat. no. 35050-061) 0.05% (wt/vol) trypsin with 0.5 mM EDTA (Gibco, cat. no. 25300-054) TrypLE Express enzyme (1×), no phenol red (Thermo, cat. no. 12604-013) Mouse recombinant epidermal growth factor (Invitrogen, cat. no. PMG8043) Nicotinamide (Sigma, cat. no. N0636) [Leu15]-Gastrin I (Sigma, cat. no. G9145) A-83-01 (Tocris Bioscience, cat. no. 2939) SB202190 (Sigma, cat. no. S7067) 50× B27 supplement (Invitrogen, cat. no. 17502-044) 100× N2 supplement (Invitrogen, cat. no. 17502-048) N -acetylcysteine (Sigma, cat. no. A9165-5G) Y-27632 stock solution (Rock inhibitor, Sigma, cat. no. Y0503–5MG, 5 mM in 1× PBS, working concentration 10 μM) Recovery cell freezing medium (Invitrogen, cat. no. 12648-010) 40 μm cell strainer (Falcon, cat. no. 352340) Puromycin dihydrochloride solution (10 mg/mL in H 2 O, Sigma, cat. no. P9620-10ML) Hygromycin-B solution (50 mg/mL in H 2 O, Sigma, cat. no. H5527-250MG) Sodium glycochenodeoxycholate (Sigma, cat. no. G0759-25MG) Matrigel growth factor reduced basement membrane matrix (Corning, cat. no. 356231) .. |